Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0051 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Cellulophaga lytica 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 6 2 PL17_2, PL17, PL6, PL6_1
PUL0052 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Maricaulis maris 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 10 2 PL17_2, PL17, PL6, PL6_1
PUL0053 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Stenotrophomonas maltophilia 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 8 2 PL17_2, PL17, PL6
PUL0054 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Alteromonas macleodii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 9 2 PL17_2, PL17, PL6, PL6_1
PUL0055 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Bacteroides sp. 1_1_30 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 7 2 PL17_2, PL17, PL6, PL6_1
PUL0056 sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis alginate Bacteroides eggerthii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 5 3 CE20, PL17_2, PL17, PL6, PL6_1
PUL0114 recombinant protein expression, enzyme activity assay arabinan Ruminiclostridium cellulolyticum 31198441
The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-alpha-l-arabinofuranosidases with complementary modes of action. Biotechnol Biofuels. 2019 Jun 10;12:144. doi: 10.1186/s13068-019-1483-y. eCollection 2019.
2019 degradation 14 14 CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6
PUL0442 mass spectrometry, high-performance anion-exchange chromatography, SDS-PAGE, recombinant protein expression, enzyme activity assay, substrate binding assay glucomannan/chitin Chitinophaga pinensis DSM 2588 28069559, 37493618
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. A polysaccharide utilization locus from Chitinophaga pinensis simultaneously targets chitin and beta-glucans found in fungal cell walls. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. mSphere. 2023 Aug 24;8(4):e0024423. doi: 10.1128/msphere.00244-23. Epub 2023 Jul 26.
2017 Mar 6,2023 Aug 24 degradation 9 3 GH16_3, CBM6, GH18, CBM6, GH18, GH18, CBM5
PUL0445 recombinant protein expression, thin-layer chromatography, enzyme activity assay alginate Sphingomonas sp. 10913091
Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000.
2000 Aug degradation 8 2 PL15_1, PL5, PL7
PUL0460 recombinant protein expression, RT-PCR, enzyme activity assay agar Paraglaciecola hydrolytica S66 29774012
A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.
2018 degradation 23 6 CE2, GH2, GH29, GH50, GH63, GH86, GH86, CBM6
PUL0485 growth assay, qRT-PCR, enzyme activity assay, affinity gel electrophoresis, crystallization, recombinant protein expression starch Flavobacterium johnsoniae 19717629, 37269952, 38661728
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Bacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria. Structural insights into alpha-(1-->6)-linkage preference of GH97 glucodextranase from Flavobacterium johnsoniae. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. J Biol Chem. 2023 Jul;299(7):104885. doi: 10.1016/j.jbc.2023.104885. Epub 2023 Jun 2. FEBS J. 2024 Jul;291(14):3267-3282. doi: 10.1111/febs.17139. Epub 2024 Apr 25.
2009 Nov,2023 Jul,2024 Jul degradation 9 5 GH27, CBM13, GH31, GH65, GH66, GH97
PUL0632 recombinant protein expression fructan Roseburia inulinivorans DSM 16841 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 GH32
PUL0633 recombinant protein expression fructan Roseburia faecis M72 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 5 1 GH32
PUL0634 recombinant protein expression fructan Eubacterium rectale ATCC 33656 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 6 1 GH32
PUL0635 recombinant protein expression fructan Coprococcus eutactus JCM 31265 33439065
Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503.
2021 Jan-Dec degradation 7 1 GH32
Showing 1 to 15 of 58 entries
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