Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390, 32585009
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL17_2, PL17, PL7
PUL0216 Western Blot, enzyme activity assay, RT-PCR, microarray alginate Sphingomonas sp. 24816607
Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9.
2014 Jul degradation 10 3 AA2, PL15_1, PL5, PL7
PUL0235 proteome fractionation, mass spectrometry, target decoy database analysis alginate Gramella forsetii 24522261
Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13.
2014 Jul degradation 20 6 PL17_2, PL17, PL6, PL6_1, PL7, PL7_5
PUL0445 recombinant protein expression, thin-layer chromatography, enzyme activity assay alginate Sphingomonas sp. 10913091
Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000.
2000 Aug degradation 8 2 PL15_1, PL5, PL7
PUL0655 sequence homology analysis alginate Maribacter dokdonensis 62-1 33912144
CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.
2021 degradation 10 2 PL7
PUL0691 recombinant protein expression, qRT-PCR alginate Vibrio pelagius WXL662 36409133
Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-Degrading Vibrio Strain and Its Degradation Strategy. Appl Environ Microbiol. 2022 Dec 13;88(23):e0138922. doi: 10.1128/aem.01389-22. Epub 2022 Nov 21.
2022 Dec 13 degradation 12 2 CBM32, PL7_5, PL7, PL7
PUL0715 clone and expression, enzyme activity assay, ultra-performance liquid chromatography–mass spectrometry(UPLC-MS) alginate Wenyingzhuangia fucanilytica CZ1127 37540808
A repertoire of alginate lyases in the alginate polysaccharide utilization loci of marine bacterium Wenyingzhuangia fucanilytica: biochemical properties and action pattern. J Sci Food Agric. 2024 Jan 15;104(1):134-140. doi: 10.1002/jsfa.12898. Epub 2023 Aug 23.
2024 Jan 15 degradation 12 4 PL17_2, PL17, PL6, PL6_1, PL7
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