PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0027 | Northern Blot, gene deletion mutant and growth assay | alginate | Azotobacter vinelandii | 10352233 Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene. 1999 May 31;232(2):217-22. doi: 10.1016/s0378-1119(99)00119-5. |
1999 May 31 | biosynthesis | 5 | 1 | PL5_1 |
| PUL0051 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Cellulophaga lytica | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 6 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0052 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Maricaulis maris | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 10 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0053 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Stenotrophomonas maltophilia | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 8 | 2 | PL17_2, PL17, PL6 |
| PUL0054 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Alteromonas macleodii | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 9 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0055 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides sp. 1_1_30 | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 7 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0056 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides eggerthii | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 5 | 3 | CE20, PL17_2, PL17, PL6, PL6_1 |
| PUL0126 | growth assay, sequence homology analysis | alginate | Alteromonas sp. 76-1 | 30936857 Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019. |
2019 | degradation | 8 | 2 | PL6, PL6_1, PL7_5 |
| PUL0127 | growth assay, sequence homology analysis | alginate | Alteromonas sp. 76-1 | 30936857 Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019. |
2019 | degradation | 12 | 4 | CBM32, PL7_5, PL6_3, PL6, PL6_1, PL7_5 |
| PUL0150 | sequence homology analysis | alginate | Pseudoalteromonas carrageenovora | 30524390 Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. |
2018 | degradation | 14 | 3 | PL17_2, PL17, PL6_3, PL6, PL6_1 |
| PUL0151 | sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay | alginate | Zobellia galactanivorans | 30524390, 32585009 Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533. |
2018,2020 Aug 20 | degradation | 12 | 2 | PL17_2, PL17, PL7 |
| PUL0152 | sequence homology analysis | alginate | Pseudoalteromonas atlantica | 30524390 Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. |
2018 | degradation | 8 | 1 | PL6, PL6_1 |
| PUL0155 | enzyme activity assay | alginate | Agrobacterium fabrum | 16545947 A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase. Res Microbiol. 2006 Sep;157(7):642-9. doi: 10.1016/j.resmic.2006.02.006. Epub 2006 Mar 2. |
2006 Sep | degradation | 8 | 1 | PL15_1 |
| PUL0199 | enzyme activity assay, liquid chromatography and mass spectrometry | alginate | Saccharophagus degradans | 26458373 Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis. Mar Biotechnol (NY). 2016 Feb;18(1):15-23. doi: 10.1007/s10126-015-9667-3. Epub 2015 Oct 12. |
2016 Feb | degradation | 17 | 6 | CBM16, CBM32, PL18, PL17_2, PL17, PL6, PL6, PL6_1, PL7_5 |
| PUL0216 | Western Blot, enzyme activity assay, RT-PCR, microarray | alginate | Sphingomonas sp. | 24816607 Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9. |
2014 Jul | degradation | 10 | 3 | AA2, PL15_1, PL5, PL7 |
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