| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0230 | RT-PCR, enzyme activity assay, clone, enzyme kinetic analysis, thin-layer chromatography, crystallization | starch | Lactobacillus acidophilus | 22685275, 32444471 Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus. J Bacteriol. 2012 Aug;194(16):4249-59. doi: 10.1128/JB.00622-12. Epub 2012 Jun 8. Appl Environ Microbiol. 2020 Jul 20;86(15):e00661-20. doi: 10.1128/AEM.00661-20. Print 2020 Jul 20. |
2012 Aug,2020 Jul 20 | degradation | 12 | 3 | CBM34, GH13_20, GH13_31, GH65 |
| PUL0236 | proteome fractionation, mass spectrometry, target decoy database analysis | alpha-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 13 | 4 | GH13, GH13_46, GH13_7, GH65 |
| PUL0436 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 8 | 3 | CBM32, CBM32, GH13_46, GH65 |
| PUL0485 | growth assay, qRT-PCR, enzyme activity assay, affinity gel electrophoresis, crystallization, recombinant protein expression | starch | Flavobacterium johnsoniae | 19717629, 37269952, 38661728 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Bacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria. Structural insights into alpha-(1-->6)-linkage preference of GH97 glucodextranase from Flavobacterium johnsoniae. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. J Biol Chem. 2023 Jul;299(7):104885. doi: 10.1016/j.jbc.2023.104885. Epub 2023 Jun 2. FEBS J. 2024 Jul;291(14):3267-3282. doi: 10.1111/febs.17139. Epub 2024 Apr 25. |
2009 Nov,2023 Jul,2024 Jul | degradation | 9 | 5 | GH27, CBM13, GH31, GH65, GH66, GH97 |
| PUL0611 | liquid chromatography and mass spectrometry | alpha-glucan | Winogradskyella sp. isolate Bin3 | 32071270 Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19. |
2020 Feb 18 | degradation | 14 | 7 | CE1, GH13_19, GH13_38, GH13_46, GH31, GH65, GH97 |
| PUL0612 | liquid chromatography and mass spectrometry | alpha-glucan | Muricauda sp. isolate Bin2 | 32071270 Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19. |
2020 Feb 18 | degradation | 11 | 2 | GH13_38, GH65 |
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