Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0230 RT-PCR, enzyme activity assay, clone, enzyme kinetic analysis, thin-layer chromatography, crystallization starch Lactobacillus acidophilus 22685275, 32444471
Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus. J Bacteriol. 2012 Aug;194(16):4249-59. doi: 10.1128/JB.00622-12. Epub 2012 Jun 8. Appl Environ Microbiol. 2020 Jul 20;86(15):e00661-20. doi: 10.1128/AEM.00661-20. Print 2020 Jul 20.
2012 Aug,2020 Jul 20 degradation 12 3 CBM34, GH13_20, GH13_31, GH65
PUL0236 proteome fractionation, mass spectrometry, target decoy database analysis alpha-glucan Gramella forsetii 24522261
Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13.
2014 Jul degradation 13 4 GH13, GH13_46, GH13_7, GH65
PUL0436 mass spectrometry, high-performance anion-exchange chromatography glucomannan Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 8 3 CBM32, CBM32, GH13_46, GH65
PUL0485 growth assay, qRT-PCR, enzyme activity assay, affinity gel electrophoresis, crystallization, recombinant protein expression starch Flavobacterium johnsoniae 19717629, 37269952, 38661728
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Bacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria. Structural insights into alpha-(1-->6)-linkage preference of GH97 glucodextranase from Flavobacterium johnsoniae. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28. J Biol Chem. 2023 Jul;299(7):104885. doi: 10.1016/j.jbc.2023.104885. Epub 2023 Jun 2. FEBS J. 2024 Jul;291(14):3267-3282. doi: 10.1111/febs.17139. Epub 2024 Apr 25.
2009 Nov,2023 Jul,2024 Jul degradation 9 5 GH27, CBM13, GH31, GH65, GH66, GH97
PUL0611 liquid chromatography and mass spectrometry alpha-glucan Winogradskyella sp. isolate Bin3 32071270
Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19.
2020 Feb 18 degradation 14 7 CE1, GH13_19, GH13_38, GH13_46, GH31, GH65, GH97
PUL0612 liquid chromatography and mass spectrometry alpha-glucan Muricauda sp. isolate Bin2 32071270
Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture. mBio. 2020 Feb 18;11(1):e03261-19. doi: 10.1128/mBio.03261-19.
2020 Feb 18 degradation 11 2 GH13_38, GH65
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