PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
---|---|---|---|---|---|---|---|---|---|
PUL0078 | enzyme activity assay | xylan | Caldicellulosiruptor sp. Rt8B.4 | 8920183 Cloning, sequencing and overexpression in Escherichia coli of a xylanase gene, xynA from the thermophilic bacterium Rt8B.4 genus Caldicellulosiruptor. Appl Microbiol Biotechnol. 1996 Mar;45(1-2):86-93. doi: 10.1007/s002530050653. |
1996 Mar | degradation | 6 | 1 | CBM22, CBM22, GH10 |
PUL0114 | recombinant protein expression, enzyme activity assay | arabinan | Ruminiclostridium cellulolyticum | 31198441 The xyl-doc gene cluster of Ruminiclostridium cellulolyticum encodes GH43- and GH62-alpha-l-arabinofuranosidases with complementary modes of action. Biotechnol Biofuels. 2019 Jun 10;12:144. doi: 10.1186/s13068-019-1483-y. eCollection 2019. |
2019 | degradation | 14 | 14 | CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6 |
PUL0224 | RT-PCR, qRT-PCR, ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 23418511, 20013800 A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2013,2010 Feb | degradation | 16 | 14 | CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6 |
PUL0322 | liquid chromatography and mass spectrometry | cellulose | Caldicellulosiruptor danielii | 29475869 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. |
2018 May 1 | degradation | 19 | 12 | CBM22, CBM22, GH10, CBM3, CBM3, GH5_1, CBM66, PL3_1, CBM66, PL9_1, GH10, CBM3, GH12, GH48, GH5_8, CBM3, CBM3, GH44, GH74, GH74, GH74, GH74, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH5_8, GT39, PL11, CBM3 |
PUL0390 | enzyme activity assay | xylan | Thermotoga maritima | 21255309 Hyperthermostable acetyl xylan esterase. Microb Biotechnol. 2010 Jan;3(1):84-92. doi: 10.1111/j.1751-7915.2009.00150.x. Epub 2009 Sep 18. |
2010 Jan | degradation | 24 | 6 | CBM22, CBM22, CBM22, GH10, CBM9, CBM9, CE7, GH10, GH3, GH67 |
PUL0642 | recombinant protein expression | starch | Roseburia intestinalis L1-82 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 11 | 6 | CBM61, GH53, CBM86, CBM22, GH10, CBM9, GH13_18, GH13_31, GH32, GH36 |
PUL0694 | recombinant protein expression, SDS-PAGE, HPLC | xylan | Caldicellulosiruptor bescii DSM 6725 | 36218355, 34060910 Biochemical and Regulatory Analyses of Xylanolytic Regulons in Caldicellulosiruptor bescii Reveal Genus-Wide Features of Hemicellulose Utilization. Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii. Appl Environ Microbiol. 2022 Nov 8;88(21):e0130222. doi: 10.1128/aem.01302-22. Epub 2022 Oct 11. mSystems. 2021 Jun 29;6(3):e0134520. doi: 10.1128/mSystems.01345-20. Epub 2021 Jun 1. |
2022 Nov 8,2021 Jun 29 | degradation | 14 | 6 | CBM22, CBM22, GH10, CE1, GH10, GH39, GH43_10, CBM22, CBM91, GH43_16, CBM6 |
PUL0695 | recombinant protein expression, SDS-PAGE, HPLC | xylan | Caldicellulosiruptor bescii DSM 6725 | 36218355, 34060910 Biochemical and Regulatory Analyses of Xylanolytic Regulons in Caldicellulosiruptor bescii Reveal Genus-Wide Features of Hemicellulose Utilization. Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii. Appl Environ Microbiol. 2022 Nov 8;88(21):e0130222. doi: 10.1128/aem.01302-22. Epub 2022 Oct 11. mSystems. 2021 Jun 29;6(3):e0134520. doi: 10.1128/mSystems.01345-20. Epub 2021 Jun 1. |
2022 Nov 8,2021 Jun 29 | degradation | 5 | 1 | CBM22, CBM22, GH10 |
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