PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0007 | sequence homology analysis | galactan | Leuconostoc gelidum | 27274361 Complete genome sequence of Leuconostoc gelidum subsp. gasicomitatum KG16-1, isolated from vacuum-packaged vegetable sausages. Stand Genomic Sci. 2016 Jun 7;11:40. doi: 10.1186/s40793-016-0164-8. eCollection 2016. |
2016 | degradation | 8 | 2 | GH42, GH53 |
| PUL0209 | enzyme activity assay, gene deletion mutant and growth assay | galactan | Dickeya dadantii | 17644603 Characterization of the Erwinia chrysanthemi Gan locus, involved in galactan catabolism. J Bacteriol. 2007 Oct;189(19):7053-61. doi: 10.1128/JB.00845-07. Epub 2007 Jul 20. |
2007 Oct | degradation | 9 | 2 | GH42, GH53 |
| PUL0282 | enzyme activity assay | galactan | Geobacillus stearothermophilus | 23216604 Functional characterization of the galactan utilization system of Geobacillus stearothermophilus. FEBS J. 2013 Feb;280(3):950-64. doi: 10.1111/febs.12089. Epub 2013 Jan 7. |
2013 Feb | degradation | 7 | 2 | CBM61, GH53, CBM61, GH42 |
| PUL0283 | microarray, high-performance anion-exchange chromatography, liquid chromatography and mass spectrometry | galactooligosaccharide | Bifidobacterium breve | 23199239 Transcriptional and functional characterization of genetic elements involved in galacto-oligosaccharide utilization by Bifidobacterium breve UCC2003. Microb Biotechnol. 2013 Jan;6(1):67-79. doi: 10.1111/1751-7915.12011. Epub 2012 Dec 2. |
2013 Jan | degradation | 6 | 2 | GH42, GH53, CBM61 |
| PUL0297 | gene trait matching exercise | galactan | Bifidobacterium longum | 29310579 Gene-trait matching across the Bifidobacterium longum pan-genome reveals considerable diversity in carbohydrate catabolism among human infant strains. BMC Genomics. 2018 Jan 8;19(1):33. doi: 10.1186/s12864-017-4388-9. |
2018 Jan 8 | degradation | 9 | 2 | GH42, GH53, CBM61 |
| PUL0304 | RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay | galactan | Bacteroides thetaiotaomicron | 29255254 Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2018 Feb | degradation | 7 | 2 | GH2, GH53 |
| PUL0306 | RT-qPCR, isothermal titration calorimetry (ITC), enzyme activity assay, gene deletion mutant and growth assay | pectin | Bacteroides ovatus | 29255254 Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2018 Feb | degradation | 7 | 3 | GH147, GH2, GH53 |
| PUL0323 | fitness contribution assay, insertion sequencing | galactan | Bacillus subtilis subsp. subtilis | 28617843 Characterization of the regulation of a plant polysaccharide utilization operon and its role in biofilm formation in Bacillus subtilis. PLoS One. 2017 Jun 15;12(6):e0179761. doi: 10.1371/journal.pone.0179761. eCollection 2017. |
2017 | degradation | 6 | 2 | GH42, GH53 |
| PUL0427 | microarray | pectin | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 6 | 2 | GH147, GH53 |
| PUL0534 | RNA-seq | pectin | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 9 | 5 | GH13_10, GH133, GH147, GH2, GH53 |
| PUL0566 | microarray, qPCR | pectin | Bacteroides ovatus | 22205877, 29255254 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18. |
2011 Dec,2018 Feb | degradation | 7 | 3 | GH147, GH2, GH53 |
| PUL0642 | recombinant protein expression | starch | Roseburia intestinalis L1-82 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 11 | 6 | CBM61, GH53, CBM86, CBM22, GH10, CBM9, GH13_18, GH13_31, GH32, GH36 |
| PUL0768 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 7 | 3 | GH2, GH53, PL1, CBM77 |
Copyright 2020 © YIN LAB, UNL. All rights reserved. Designed by Catie Ausland and Jinfang Zheng. Maintained by Yanbin Yin.