Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0010	enzyme activity assay, liquid chromatography and mass spectrometry	xylan	Geobacillus thermodenitrificans	28616644 Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.	2017 Aug	degradation	42	8	CE4, GH10, GH39, GH43_11, CBM91, GH51_1, GH52, GH67
PUL0542	binding assay	xylan	Geobacillus stearothermophilus	10368143 The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6. J Bacteriol. 1999 Jun;181(12):3695-704. doi: 10.1128/JB.181.12.3695-3704.1999.	1999 Jun	degradation	29	7	CE4, GH10, GH39, GH43_11, CBM91, GH52, GH67
PUL0602	sequence homology analysis	xylan	Parageobacillus thermoglucosidasius	26442136 Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park. Stand Genomic Sci. 2015 Oct 5;10:73. doi: 10.1186/s40793-015-0031-z. eCollection 2015.	2015	degradation	26	6	CE4, GH10, GH39, GH52, GH67
PUL0650	enzyme activity assay, high-performance anion-exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay	arabinogalactan	Bifidobacterium longum JCM 7052	33674431 Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27.	2021 Apr 27	degradation	7	2	GH36, GH39, CBM35
PUL0694	recombinant protein expression, SDS-PAGE, HPLC	xylan	Caldicellulosiruptor bescii DSM 6725	36218355, 34060910 Biochemical and Regulatory Analyses of Xylanolytic Regulons in Caldicellulosiruptor bescii Reveal Genus-Wide Features of Hemicellulose Utilization. Transcriptional Regulation of Plant Biomass Degradation and Carbohydrate Utilization Genes in the Extreme Thermophile Caldicellulosiruptor bescii. Appl Environ Microbiol. 2022 Nov 8;88(21):e0130222. doi: 10.1128/aem.01302-22. Epub 2022 Oct 11. mSystems. 2021 Jun 29;6(3):e0134520. doi: 10.1128/mSystems.01345-20. Epub 2021 Jun 1.	2022 Nov 8,2021 Jun 29	degradation	14	6	CBM22, CBM22, GH10, CE1, GH10, GH39, GH43_10, CBM22, CBM91, GH43_16, CBM6
PUL0743	gene mutant, SDS-PAGE, Western Blot, recombinant protein expression, thermal shift assay (TSA), isothermal titration calorimetry (ITC), HPAEC-PAD, RT-qPCR, fluorescence measurements	xylan	Ruminiclostridium cellulolyticum H10	36403068, 38789996 Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow. Intracellular removal of acetyl, feruloyl and p-coumaroyl decorations on arabinoxylo-oligosaccharides imported from lignocellulosic biomass degradation by Ruminiclostridium cellulolyticum. Biotechnol Biofuels Bioprod. 2022 Nov 19;15(1):127. doi: 10.1186/s13068-022-02225-8. Microb Cell Fact. 2024 May 24;23(1):151. doi: 10.1186/s12934-024-02423-z.	2022 Nov 19,2024 May 24	degradation	13	6	CE1, CE20, CE20, GH39, GH43_10, CBM91, GH51_1, GH8
PUL0752	thin-layer chromatography, HPAEC-PAD, recombinant protein expression, in vitro assimilation	fructo-disaccharide	Blautia parvula NBRC 113351	39500763 Degradation mechanism of difructose dianhydride III in Blautia species. Appl Microbiol Biotechnol. 2024 Nov 5;108(1):502. doi: 10.1007/s00253-024-13346-5.	2024 Nov 5	degradation	10	3	GH32, GH39, GH91
PUL0795	RNA-seq, recombinant protein expression, growth assay	xyloglucan	Flavobacterium johnsoniae UW101	39913342 Metabolism of hemicelluloses by root-associated Bacteroidota species. ISME J. 2025 Jan 2;19(1):wraf022. doi: 10.1093/ismejo/wraf022.	2025 Jan 2	degradation	12	8	CE20, CE20, GH2, GH3, GH31_3, GH39, GH5_4, GH95, GH97