Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0083 label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay cellulose Ruminiclostridium papyrosolvens 31338125
Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019.
2019 degradation 12 11 CBM3, cohesin, cohesin, cohesin, cohesin, cohesin, cohesin, CBM4, GH9, GH48, GH5_1, GH5_17, GH5_7, GH8, GH9, GH9, CBM3
PUL0262 RNA-seq xylan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 12 6 CE1, CE6, GH95, GH10, GH5_21, GH8
PUL0311 enzyme activity assay cellulose Escherichia coli 31455320
Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4. BMC Biotechnol. 2019 Aug 27;19(1):63. doi: 10.1186/s12896-019-0556-0.
2019 Aug 27 degradation 4 2 GH8, GT2
PUL0345 qRT-PCR, enzyme activity assay xylan Bacteroides intestinalis 27681607
Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Sci Rep. 2016 Sep 29;6:34360. doi: 10.1038/srep34360.
2016 Sep 29 degradation 31 13 CE1, CE20, CE20, CE6, GH95, GH10, GH10, GH43_12, CBM91, GH115, GH35, GH43_1, GH5_21, GH67, GH8
PUL0385 ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography cellulose Ruminiclostridium cellulolyticum 20013800, 8936327
Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013.
2010 Feb,1996 Apr degradation 12 10 CBM4, GH9, GH48, GH5_1, GH5_17, GH8, GH9, GH9, CBM3, PL11
PUL0480 growth assay xylan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 4 GH10, GH16, GH3, GH8
PUL0648 high-performance anion-exchange chromatography, substrate binding assay, thin-layer chromatography, NMR, mass spectrometry, crystallization xylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 21 CE1, CE1, CE1, CE20, CE20, CE6, GH10, GH115, GH146, GH31_4, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH43_29, GH43_29, CBM6, GH51_2, GH67, GH8, GH97
PUL0669 clone, high-performance anion-exchange chromatography, enzymatic product analysis xylan Bacteroides eggerthii 1_2_48FAA 34480044
Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii. Sci Rep. 2021 Sep 3;11(1):17662. doi: 10.1038/s41598-021-96659-z.
2021 Sep 3 degradation 26 15 CE1, CE15, GH8, CE20, CE20, CE6, GH10, GH115, GH31_4, GH35, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH5_21, GH67, GH95, GH97
PUL0705 fluorophore-assisted carbohydrate electrophoresis (FACE), dinitrosalicylic acid-assay (DNS-assay), HPLC, clone and expression xylan Flavimarina sp. Hel_I_48 37121608
Marine Bacteroidetes enzymatically digest xylans from terrestrial plants. Environ Microbiol. 2023 Sep;25(9):1713-1727. doi: 10.1111/1462-2920.16390. Epub 2023 Apr 30.
2023 Sep degradation 14 8 CE6, CE1, CE1, GH10, GH43_10, CBM91, GH43_12, CBM91, GH8, GH95, GH97
PUL0743 gene mutant, SDS-PAGE, Western Blot, recombinant protein expression, thermal shift assay (TSA), isothermal titration calorimetry (ITC), HPAEC-PAD, RT-qPCR, fluorescence measurements xylan Ruminiclostridium cellulolyticum H10 36403068, 38789996
Selfish uptake versus extracellular arabinoxylan degradation in the primary degrader Ruminiclostridium cellulolyticum, a new string to its bow. Intracellular removal of acetyl, feruloyl and p-coumaroyl decorations on arabinoxylo-oligosaccharides imported from lignocellulosic biomass degradation by Ruminiclostridium cellulolyticum. Biotechnol Biofuels Bioprod. 2022 Nov 19;15(1):127. doi: 10.1186/s13068-022-02225-8. Microb Cell Fact. 2024 May 24;23(1):151. doi: 10.1186/s12934-024-02423-z.
2022 Nov 19,2024 May 24 degradation 13 6 CE1, CE20, CE20, GH39, GH43_10, CBM91, GH51_1, GH8
PUL0763 RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography Hemicellulose Segatella copri DSM 18205 39636128
Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5.
2025 Jan 31 degradation 5 3 CE6, CE1, GH31_4, GH43_2, CBM6, GH8
PUL0792 enzyme activity assay, recombinant protein expression, RNA-seq xylan Bifidobacterium pseudocatenulatum strain YIT11952 37938239
Xylan utilisation promotes adaptation of Bifidobacterium pseudocatenulatum to the human gastrointestinal tract. ISME Commun. 2021 Oct 28;1(1):62. doi: 10.1038/s43705-021-00066-4.
2021 Oct 28 degradation 15 5 CE20, GH10, CBM9, GH120, GH43_11, CBM91, GH8
Showing 1 to 12 of 12 entries
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