PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0040 | Northern Blot, enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 12896991, 1398087, 11844767 A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome. J Bacteriol. 2003 Aug;185(16):4727-33. doi: 10.1128/JB.185.16.4727-4733.2003. Gene. 1992 Sep 21;119(1):17-28. doi: 10.1016/0378-1119(92)90062-t. J Bacteriol. 2002 Mar;184(5):1378-84. doi: 10.1128/JB.184.5.1378-1384.2002. |
2003 Aug,1992 Sep 21,2002 Mar | degradation | 6 | 6 | GH5_1, GH5_17, GH9, GH9, CBM3, PL11 |
| PUL0083 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | cellulose | Ruminiclostridium papyrosolvens | 31338125 Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 12 | 11 | CBM3, cohesin, cohesin, cohesin, cohesin, cohesin, cohesin, CBM4, GH9, GH48, GH5_1, GH5_17, GH5_7, GH8, GH9, GH9, CBM3 |
| PUL0320 | liquid chromatography and mass spectrometry, mass spectrometry, target decoy database analysis | cellulose | Caldicellulosiruptor bescii | 29475869, 29588665, 21227922 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate. Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. Biotechnol Biofuels. 2018 Mar 23;11:80. doi: 10.1186/s13068-018-1076-1. eCollection 2018. Nucleic Acids Res. 2011 Apr;39(8):3240-54. doi: 10.1093/nar/gkq1281. Epub 2011 Jan 11. |
2018 May 1,2018,2011 Apr | degradation | 19 | 10 | CBM66, PL3_1, CBM66, PL9_1, GH10, CBM3, CBM3, GH48, GH5_8, CBM3, CBM3, CBM3, GH5_1, GH5_8, CBM3, CBM3, GH44, GH74, GH74, GH74, GH74, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH5_8, GT39, PL11, CBM3 |
| PUL0322 | liquid chromatography and mass spectrometry | cellulose | Caldicellulosiruptor danielii | 29475869 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. |
2018 May 1 | degradation | 19 | 12 | CBM22, CBM22, GH10, CBM3, CBM3, GH5_1, CBM66, PL3_1, CBM66, PL9_1, GH10, CBM3, GH12, GH48, GH5_8, CBM3, CBM3, GH44, GH74, GH74, GH74, GH74, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH5_8, GT39, PL11, CBM3 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800, 8936327 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013. |
2010 Feb,1996 Apr | degradation | 12 | 10 | CBM4, GH9, GH48, GH5_1, GH5_17, GH8, GH9, GH9, CBM3, PL11 |
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