Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0225	RT-PCR	agarose	Bacteroides plebeius	23150581 Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19786-91. doi: 10.1073/pnas.1211002109. Epub 2012 Nov 12.	2012 Nov 27	degradation	36	12	GH105, GH154, GH117, GH117, GH16_12, GH16_14, GH16_16, GH2, GH29, GH50, GH86
PUL0393	enzyme activity assay, analysis of reaction products	galactan	Microbulbifer thermotolerans	20686828 Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94T, and insight into the unusual catalytic mechanism. Mar Biotechnol (NY). 2011 Jun;13(3):411-22. doi: 10.1007/s10126-010-9312-0. Epub 2010 Aug 5.	2011 Jun	degradation	5	2	CBM6, CBM6, GH86, GH86, GH16_16, CBM6
PUL0459	RNA-seq, analysis of reaction products, enzyme activity assay, thin-layer chromatography, liquid chromatography, mass spectrometry	agarose	Colwellia echini A3	31915221, 33811026 A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19. Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26.	2020 Jan 8,2021 May 26	degradation	45	9	GH117, GH117, GH2, GH29, GH50, GH86, GH96
PUL0460	recombinant protein expression, RT-PCR, enzyme activity assay	agar	Paraglaciecola hydrolytica S66	29774012 A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.	2018	degradation	23	6	CE2, GH2, GH29, GH50, GH63, GH86, GH86, CBM6
PUL0651	enzyme activity assay, NMR	agarose	Gilvimarinus chinensis DSM 19667	33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19.	2021 Aug 1	degradation	63	15	CBM6, CBM6, CBM6, GH86, GH86, CE1, GH117, GH127, GH16_16, CBM13, GH16_16, CBM6, CBM6, GH16_3, GH167, GH2, GH50, GH86
PUL0703	enzyme activity assay, recombinant protein expression, thin-layer chromatography	agarose	Aquimarina sp. ERC-38	37002465 Agarolytic Pathway in the Newly Isolated Aquimarina sp. Bacterial Strain ERC-38 and Characterization of a Putative beta-agarase. Mar Biotechnol (NY). 2023 Apr;25(2):314-327. doi: 10.1007/s10126-023-10206-7. Epub 2023 Apr 1.	2023 Apr	degradation	36	10	CE1, GH117, GH117, GH16_15, GH16_16, GH16_16, CBM6, GH2, GH82, GH86, GH86, GH86, CBM6
PUL0706	RNA-seq, growth assay	agar	Pseudoalteromonas atlantica T6c	37265394 Constructing Marine Bacterial Metabolic Chassis for Potential Biorefinery of Red Algal Biomass and Agaropectin Wastes. ACS Synth Biol. 2023 Jun 16;12(6):1782-1793. doi: 10.1021/acssynbio.3c00063. Epub 2023 Jun 2.	2023 Jun 16	degradation	43	15	CE20, CE20, GH117, GH117, GH140, GH16_12, GH16_14, GH2, GH29, GH3, GH43_12, CBM91, GH43_2, CBM6, GH86
PUL0708	RNA-seq, growth assay	agar	Pseudoalteromonas atlantica T6c	37265394 Constructing Marine Bacterial Metabolic Chassis for Potential Biorefinery of Red Algal Biomass and Agaropectin Wastes. ACS Synth Biol. 2023 Jun 16;12(6):1782-1793. doi: 10.1021/acssynbio.3c00063. Epub 2023 Jun 2.	2023 Jun 16	degradation	41	5	CE1, GH13_38, GH31, GH86