Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0008 enzyme activity assay, qPCR, thin-layer chromatography, substrate binding assay fructan Bacteroides thetaiotaomicron 28103254, 25841008
A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.
2017,2015 Jun 15 degradation 12 3 GH32
PUL0024 enzyme activity assay, qPCR, carbohydrate binding assay fructan uncultured bacterium 31915220
Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19.
2020 Jan 8 degradation 12 1 GH32
PUL0026 qPCR, Western Blot, RNA-seq, enzyme activity assay ribose Bacteroides thetaiotaomicron 31901520
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner. Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.
2020 Jan 8 degradation 8 1 GH35
PUL0028 microarray, qPCR, enzyme activity assay mucin [Ruminococcus] gnavus 24204617
Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent. PLoS One. 2013 Oct 25;8(10):e76341. doi: 10.1371/journal.pone.0076341. eCollection 2013.
2013 degradation 14 4 CBM40, GH33, GH1, GH140, GH177
PUL0068 enzyme activity assay, electrophoretic mobility shift assay raffinose Escherichia coli 8277949
Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli. Mol Gen Genet. 1994 Jan;242(1):90-9. doi: 10.1007/BF00277352.
1994 Jan degradation 4 2 GH32, GH36
PUL0082 electrophoretic mobility shift assay, enzyme activity assay melibiose Bacillus subtilis 31138628
The melREDCA Operon Encodes a Utilization System for the Raffinose Family of Oligosaccharides in Bacillus subtilis. J Bacteriol. 2019 Jul 10;201(15):e00109-19. doi: 10.1128/JB.00109-19. Print 2019 Aug 1.
2019 Aug 1 degradation 6 2 CE19, GH4
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390, 32585009
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL17_2, PL17, PL7
PUL0161 mass spectrometry, sequence homology analysis, gene deletion mutant and growth assay, microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 30246424, 25567280, 18996345
Alpha- and beta-mannan utilization by marine Bacteroidetes. Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2018 Nov,2015 Jan 8,2008 Nov 13 degradation 26 6 GH125, GH67, GH76, GH92, GH97
PUL0163 mass spectrometry, sequence homology analysis, microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 30246424, 18996345
Alpha- and beta-mannan utilization by marine Bacteroidetes. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2018 Nov,2008 Nov 13 degradation 21 9 GH125, GH130_3, GH38, CBM32, GH76, GH92, GT32
PUL0189 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 17 9 CBM67, GH78, CBM67, GH78, GH33, CE19, GH140, GH28, GH43_18, GH92, GH95, PL1_2
PUL0190 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 15 6 GH146, GH43_29, GH43_4, GH51_1, GH51_2
PUL0191 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 11 5 CE12, CE8, CE8, GH105, PL1_2
PUL0192 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 27 14 CE12, CE12, CE12, GH105, GH106, GH2, GH28, GH42, GH43_18, GH43_34, CBM32, PL11, PL11_1, PL26
PUL0193 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 30 8 CE20, GH105, GH117, GH117, GH2, GH28, PL11
PUL0204 qPCR, thin-layer chromatography, substrate binding assay starch Bacteroides thetaiotaomicron 25841008, 18996345
Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2015 Jun 15,2008 Nov 13 degradation 7 3 GH13_36, GH13_46, GH97
Showing 1 to 15 of 62 entries
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