Browse dbCAN-PUL Entries

PULID	Characterization Method(s)	Substrate	Organism	Publication	Publish Date	Type	Num Genes	Num CAZymes	CazyFamily
PUL0101	sequence homology analysis	host glycan	Bacteroides plebeius	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	19	4	CBM67, GH78, GH115, GH3, GH97
PUL0102	fosmid library screen, lectin binding assay	host glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	25	6	CBM67, GH78, CBM93, GH33, CE20, CE3, GH20, GH29
PUL0103	fosmid library screen, lectin binding assay	host glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	31	2	CBM67, GH78, CBM93, GH33
PUL0104	fosmid library screen, lectin binding assay	host glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	33	2	CBM67, GH78, CBM93, GH33
PUL0105	fosmid library screen, lectin binding assay	host glycan	uncultured bacterium	31275257 Investigating Host Microbiota Relationships Through Functional Metagenomics. Front Microbiol. 2019 Jun 7;10:1286. doi: 10.3389/fmicb.2019.01286. eCollection 2019.	2019	degradation	36	5	CBM67, GH78, CBM93, GH33, GH115, GH3, GH97
PUL0189	RNA-seq, RT-PCR, qPCR	pectin	Bacteroides xylanisolvens	26920945 Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.	2016 Feb 27	degradation	17	9	CBM67, GH78, CBM67, GH78, GH33, CE19, GH140, GH28, GH43_18, GH92, GH95, PL1_2
PUL0365	RT-PCR	starch	Xanthomonas campestris pv. campestris	17311090 Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. PLoS One. 2007 Feb 21;2(2):e224. doi: 10.1371/journal.pone.0000224.	2007 Feb 21	degradation	8	4	GH13_2, GH13_23, GH78, GH97
PUL0419	microarray	pectin	Bacteroides ovatus	22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2011 Dec	degradation	11	7	CBM67, GH78, GH130_1, GH140, GH2, GH5_2, GH5_5
PUL0472	microarray, qPCR	mucin	Bacteroides thetaiotaomicron	18996345 Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.	2008 Nov 13	degradation	28	10	CBM67, GH78, CE1, CE20, CE20, GH130_3, GH2, GH38, GH43_8, GH92
PUL0482	growth assay	pectin	Flavobacterium johnsoniae	19717629 Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.	2009 Nov	degradation	27	21	CBM67, GH78, CE19, CE20, CE8, GH106, GH127, GH137, GH139, GH140, GH142, GH143, GH2, GH28, GH43_18, GH78, GH95, PL1_2, PL29
PUL0552	RNA-seq	arabinan	Bacteroides cellulosilyticus	23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.	2013	degradation	7	4	CBM67, GH78, GH143, GH142, GH43_18, PL1_2
PUL0558	gene deletion mutant and growth assay, growth assay, enzyme activity assay	pectin	Bacteroides thetaiotaomicron	28329766, 22205877 Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.	2017 Apr 6,2011 Dec	degradation	50	21	CBM67, GH78, CBM67, GH78, GH33, CE19, CE20, GH105, GH106, GH127, GH137, GH2, CBM57, CBM97, GH138, GH139, GH140, GH141, GH143, GH142, GH2, GH28, GH43_18, GH78, GH95, PL1_2
PUL0681	enzyme activity assay, NMR	pectic polysaccharide	Bacteroidaceae bacterium	35110564 Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides. Nat Commun. 2022 Feb 2;13(1):629. doi: 10.1038/s41467-022-28310-y.	2022 Feb 2	degradation	10	5	GH173, GH2, GH5_57, GH78
PUL0723	crystallization, high-performance anion-exchange chromatography, enzyme activity assay	pectin	Phocaeicola dorei DSM 17855	38179933 Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds. Appl Environ Microbiol. 2024 Jan 24;90(1):e0176823. doi: 10.1128/aem.01768-23. Epub 2024 Jan 5.	2024 Jan 24	degradation	17	12	CE12, CE15, GH105, GH106, GH106, GH28, GH2, GH28, GH42, GH43_18, GH43_34, CBM32, GH78, PL11
PUL0724	crystallization, high-performance anion-exchange chromatography, enzyme activity assay	pectin	Phocaeicola vulgatus ATCC 8482	38179933 Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds. Appl Environ Microbiol. 2024 Jan 24;90(1):e0176823. doi: 10.1128/aem.01768-23. Epub 2024 Jan 5.	2024 Jan 24	degradation	16	12	CE12, CE15, GH105, GH106, GH106, GH28, GH2, GH28, GH42, GH43_18, GH43_34, CBM32, GH78, PL11