Browse dbCAN-PUL Entries

PULID
Characterization Method(s)
Substrate
Organism
Publication
Publish Date
Type
Num Genes
Num CAZymes
CazyFamily
PUL0001 RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 15 9 CE17, CBM35inCE17, CE2, GH1, GH113, GH130_1, GH130_2, GH36
PUL0002 enzyme activity assay, Northern Blot beta-glucan Bacillus subtilis 8606172
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J Bacteriol. 1996 Apr;178(7):1971-9. doi: 10.1128/jb.178.7.1971-1979.1996.
1996 Apr degradation 2 1 GH16_21
PUL0004 enzyme activity assay, substrate binding assay beta-glucan uncultured bacterium 26827771
A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.
2016 Mar degradation 2 1 GH1
PUL0005 enzyme activity assay, crystallization beta-glucan Listeria innocua 26886583
Functional and Structural Analysis of a beta-Glucosidase Involved in beta-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 2016 Feb 17;11(2):e0148870. doi: 10.1371/journal.pone.0148870. eCollection 2016.
2016 degradation 2 2 GH3, GH94
PUL0006 enzyme activity assay galactan Geobacillus stearothermophilus 24637762
Purification, crystallization and preliminary crystallographic analysis of Gan1D, a GH1 6-phospho-beta-galactosidase from Geobacillus stearothermophilus T1. Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):225-31. doi: 10.1107/S2053230X13034778. Epub 2014 Jan 21.
2014 Feb degradation 10 1 GH1
PUL0008 enzyme activity assay, qPCR, thin-layer chromatography, substrate binding assay fructan Bacteroides thetaiotaomicron 28103254, 25841008
A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.
2017,2015 Jun 15 degradation 12 3 GH32
PUL0009 enzyme activity assay, immunoblotting, ATPase assay glycosaminoglycan Streptobacillus moniliformis 28432302
A bacterial ABC transporter enables import of mammalian host glycosaminoglycans. Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y.
2017 Apr 21 degradation 15 4 GH88, PL12_1, PL8
PUL0010 enzyme activity assay, liquid chromatography and mass spectrometry xylan Geobacillus thermodenitrificans 28616644
Synergistic hydrolysis of xylan using novel xylanases, beta-xylosidases, and an alpha-L-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2. Appl Microbiol Biotechnol. 2017 Aug;101(15):6023-6037. doi: 10.1007/s00253-017-8341-2. Epub 2017 Jun 14.
2017 Aug degradation 42 8 CE4, GH10, GH39, GH43_11, CBM91, GH51_1, GH52, GH67
PUL0012 enzyme activity assay chitin Vibrio cholerae 28683122
The nucleoid occlusion protein SlmA is a direct transcriptional activator of chitobiose utilization in Vibrio cholerae. PLoS Genet. 2017 Jul 6;13(7):e1006877. doi: 10.1371/journal.pgen.1006877. eCollection 2017 Jul.
2017 Jul degradation 11 3 GH20, GH9, GH94
PUL0018 rapid plate method growth assay, gene deletion mutant and growth assay, RT-PCR, enzyme activity assay glycosaminoglycan Streptococcus pneumoniae 22311922
Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth. Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.
2012 Apr degradation 13 3 CBM70, PL8_1, GH88, PL12_1
PUL0019 enzyme activity assay, Northern Blot beta-glucan Bacillus subtilis 8990303
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis. J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997.
1997 Jan degradation 6 1 GH4
PUL0022 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 6 1 GH1
PUL0023 RT-PCR, gene deletion mutant and growth assay, enzyme activity assay cellobiose Bacillus coagulans 30519284
Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.
2018 degradation 5 1 GH1
PUL0024 enzyme activity assay, qPCR, carbohydrate binding assay fructan uncultured bacterium 31915220
Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19.
2020 Jan 8 degradation 12 1 GH32
PUL0026 qPCR, Western Blot, RNA-seq, enzyme activity assay ribose Bacteroides thetaiotaomicron 31901520
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner. Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.
2020 Jan 8 degradation 8 1 GH35
Showing 1 to 15 of 216 entries
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