Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0001 RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 15 9 CE17, CBM35inCE17, CE2, GH1, GH113, GH130_1, GH130_2, GH36
PUL0004 enzyme activity assay, substrate binding assay beta-glucan uncultured bacterium 26827771
A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15.
2016 Mar degradation 2 1 GH1
PUL0008 enzyme activity assay, qPCR, thin-layer chromatography, substrate binding assay fructan Bacteroides thetaiotaomicron 28103254, 25841008
A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.
2017,2015 Jun 15 degradation 12 3 GH32
PUL0024 enzyme activity assay, qPCR, carbohydrate binding assay fructan uncultured bacterium 31915220
Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19.
2020 Jan 8 degradation 12 1 GH32
PUL0026 qPCR, Western Blot, RNA-seq, enzyme activity assay ribose Bacteroides thetaiotaomicron 31901520
A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner. Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.
2020 Jan 8 degradation 8 1 GH35
PUL0028 microarray, qPCR, enzyme activity assay mucin [Ruminococcus] gnavus 24204617
Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent. PLoS One. 2013 Oct 25;8(10):e76341. doi: 10.1371/journal.pone.0076341. eCollection 2013.
2013 degradation 14 4 CBM40, GH33, GH1, GH140, GH177
PUL0099 RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry beta-mannan Roseburia intestinalis 30796211
The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y.
2019 Feb 22 degradation 3 3 CBM27, GH26, CBM23, GH3
PUL0161 mass spectrometry, sequence homology analysis, gene deletion mutant and growth assay, microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 30246424, 25567280, 18996345
Alpha- and beta-mannan utilization by marine Bacteroidetes. Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2018 Nov,2015 Jan 8,2008 Nov 13 degradation 26 6 GH125, GH67, GH76, GH92, GH97
PUL0163 mass spectrometry, sequence homology analysis, microarray, qPCR alpha-mannan Bacteroides thetaiotaomicron 30246424, 18996345
Alpha- and beta-mannan utilization by marine Bacteroidetes. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2018 Nov,2008 Nov 13 degradation 21 9 GH125, GH130_3, GH38, CBM32, GH76, GH92, GT32
PUL0189 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 17 9 CBM67, GH78, CBM67, GH78, GH33, CE19, GH140, GH28, GH43_18, GH92, GH95, PL1_2
PUL0190 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 15 6 GH146, GH43_29, GH43_4, GH51_1, GH51_2
PUL0191 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 11 5 CE12, CE8, CE8, GH105, PL1_2
PUL0192 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 27 14 CE12, CE12, CE12, GH105, GH106, GH2, GH28, GH42, GH43_18, GH43_34, CBM32, PL11, PL11_1, PL26
PUL0193 RNA-seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 30 8 CE20, GH105, GH117, GH117, GH2, GH28, PL11
PUL0204 qPCR, thin-layer chromatography, substrate binding assay starch Bacteroides thetaiotaomicron 25841008, 18996345
Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2015 Jun 15,2008 Nov 13 degradation 7 3 GH13_36, GH13_46, GH97
PUL0205 qPCR, thin-layer chromatography, substrate binding assay dextran Bacteroides thetaiotaomicron 25841008
Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3.
2015 Jun 15 degradation 6 3 GH31, GH31_14, GH66
PUL0215 qPCR, enzyme activity assay xyloglucan Cellvibrio japonicus 25171165
A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus. Mol Microbiol. 2014 Oct;94(2):418-33. doi: 10.1111/mmi.12776. Epub 2014 Sep 17.
2014 Oct degradation 4 3 GH31_4, GH35, GH95
PUL0227 enzyme activity assay, substrate binding assay xylan Caldanaerobius polysaccharolyticus 22918832
Biochemical and structural insights into xylan utilization by the thermophilic bacterium Caldanaerobius polysaccharolyticus. J Biol Chem. 2012 Oct 12;287(42):34946-34960. doi: 10.1074/jbc.M112.391532. Epub 2012 Aug 22.
2012 Oct 12 degradation 10 3 CE4, GH3, GH67
PUL0291 electrophoretic mobility shift assay, qPCR human milk oligosaccharide Escherichia coli 29453395
The genes of the sulphoquinovose catabolism in Escherichia coli are also associated with a previously unknown pathway of lactose degradation. Sci Rep. 2018 Feb 16;8(1):3177. doi: 10.1038/s41598-018-21534-3.
2018 Feb 16 degradation 10 1 GH31_13
PUL0309 enzyme activity assay, substrate binding assay, isothermal titration calorimetry (ITC) arabinan Caldanaerobius polysaccharolyticus 28710263
Enzymatic Mechanism for Arabinan Degradation and Transport in the Thermophilic Bacterium Caldanaerobius polysaccharolyticus. Appl Environ Microbiol. 2017 Aug 31;83(18):e00794-17. doi: 10.1128/AEM.00794-17. Print 2017 Sep 15.
2017 Sep 15 degradation 12 6 GH127, GH146, GH27, GH43_4, GH51_1
PUL0374 microarray, qPCR melibiose Dickeya dadantii 19734309
Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937. J Bacteriol. 2009 Nov;191(22):6960-7. doi: 10.1128/JB.00594-09. Epub 2009 Sep 4.
2009 Nov degradation 3 1 GH36
PUL0376 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 9 2 GH16_3, GH18
PUL0377 microarray, qPCR, enzyme activity assay glycosaminoglycan Bacteroides thetaiotaomicron 18996345, 30262663
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. J Biol Chem. 2018 Nov 16;293(46):17906-17916. doi: 10.1074/jbc.RA118.004510. Epub 2018 Sep 27.
2008 Nov 13,2018 Nov 16 degradation 27 5 GH2, GH88, PL29, PL8_2
PUL0380 microarray, qPCR, microarray, enzyme activity assay, strcutural analysis, clone and expression mucin Bacteroides thetaiotaomicron 18996345, 16968696, 32060313
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12. Nat Commun. 2020 Feb 14;11(1):899. doi: 10.1038/s41467-020-14754-7.
2008 Nov 13,2006 Nov 24,2020 Feb 14 degradation 12 4 GH18, GH92
PUL0382 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 11 4 CBM32, GH109, GH2
PUL0383 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 5 1 GH89
PUL0384 microarray, qPCR glycosaminoglycan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 12 4 GH88, PL12_2, PL15_2
PUL0392 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 8 3 CE20, CE20, GH13_14, GH67
PUL0442 mass spectrometry, high-performance anion-exchange chromatography, SDS-PAGE, recombinant protein expression, enzyme activity assay, substrate binding assay glucomannan/chitin Chitinophaga pinensis DSM 2588 28069559, 37493618
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. A polysaccharide utilization locus from Chitinophaga pinensis simultaneously targets chitin and beta-glucans found in fungal cell walls. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. mSphere. 2023 Aug 24;8(4):e0024423. doi: 10.1128/msphere.00244-23. Epub 2023 Jul 26.
2017 Mar 6,2023 Aug 24 degradation 9 3 GH16_3, CBM6, GH18, CBM6, GH18, GH18, CBM5
PUL0463 microarray, qPCR host glycan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 13 2 CE2, GH89
PUL0464 microarray, qPCR host glycan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 16 8 CBM93, GH33, CE20, GH2, GH20, GH20, CBM32
PUL0465 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 8 2 GH18
PUL0467 microarray, qPCR, expression of recombinant proteins, RNA-seq, differential gene expression host glycan Bacteroides thetaiotaomicron 18996345, 31160824
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. Nat Microbiol. 2019 Sep;4(9):1571-1581. doi: 10.1038/s41564-019-0466-x. Epub 2019 Jun 3.
2008 Nov 13,2019 Sep degradation 14 4 GH18, GH2, GH20, GH29, CBM32
PUL0468 microarray, qPCR host glycan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 47 4 GH109, GH177, GH43_26, GH76
PUL0470 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 7 1 GH18
PUL0471 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 9 4 CBM32, GH92
PUL0472 microarray, qPCR mucin Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 28 10 CBM67, GH78, CE1, CE20, CE20, GH130_3, GH2, GH38, GH43_8, GH92
PUL0526 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 6 2 GH3, GH30_3
PUL0527 microarray, qPCR xyloglucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 16 8 GH2, GH3, GH31_4, GH43_12, CBM91, GH5_4, GH9
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH16_3, GH3
PUL0529 microarray, qPCR, RNA-seq, reducing-sugar assay, growth assay pectin Bacteroides ovatus 22205877, 39892338
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. In vitro fermentation of a purified fraction of polysaccharides from the root of Brassica rapa L. by human gut microbiota and its interaction with Bacteroides ovatus. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Food Chem. 2025 May 1;473:143109. doi: 10.1016/j.foodchem.2025.143109. Epub 2025 Jan 27.
2011 Dec,2025 May 1 degradation 27 13 CE12, CE8, CE8, GH105, GH28, GH3, GH43_10, CBM91, PL1_2
PUL0553 RT-PCR, qPCR xylan Bacteroides xylanisolvens 27142817
Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genomics. 2016 May 4;17:326. doi: 10.1186/s12864-016-2680-8.
2016 May 4 degradation 22 13 CE6, CE1, GH10, GH115, GH3, GH31_4, GH43_10, CBM91, GH43_12, CBM91, GH43_29, CBM6, GH5_21, GH95, GH97
PUL0559 gene deletion mutant and growth assay, growth assay, enzyme activity assay, microarray, qPCR pectin Bacteroides thetaiotaomicron 28329766, 18996345, 22205877, 16968696
Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Functional genomic and metabolic studies of the adaptations of a prominent adult human gut symbiont, Bacteroides thetaiotaomicron, to the suckling period. Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725. Epub 2017 Mar 22. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2006 Nov 24;281(47):36269-79. doi: 10.1074/jbc.M606509200. Epub 2006 Sep 12.
2017 Apr 6,2008 Nov 13,2011 Dec,2006 Nov 24 degradation 12 4 GH29, GH43_10, CBM91, GH43_34, CBM32, GH97
PUL0560 microarray, qPCR arabinogalactan Bacteroides thetaiotaomicron 18996345, 22205877
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2008 Nov 13,2011 Dec degradation 30 4 GH35, GH43_24, PL27
PUL0563 microarray, qPCR arabinogalactan Bacteroides thetaiotaomicron 18996345, 22205877
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2008 Nov 13,2011 Dec degradation 15 7 GH105, GH127, GH154, GH16_3, GH43_24, GH43_24, GH43_34, PL42
PUL0564 microarray, qPCR, UHPLC-MS, RNA-seq, RT-qPCR pectin Bacteroides thetaiotaomicron 18996345, 22205877, 34420703
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Discrete genetic loci in human gut Bacteroides thetaiotaomicron confer pectin metabolism. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.
2008 Nov 13,2011 Dec,2021 Nov 15 degradation 39 22 CE12, CE12, CE12, CE4, CE6, GH105, GH106, GH2, GH27, GH28, GH35, GH43_18, GH42, PL11_1, PL26, PL9, PL9_1
PUL0565 microarray, qPCR galactomannan Bacteroides ovatus 22205877, 27872187
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A beta-MANNANASE. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. J Biol Chem. 2017 Jan 6;292(1):229-243. doi: 10.1074/jbc.M116.746438. Epub 2016 Nov 21.
2011 Dec,2017 Jan 6 degradation 10 4 GH130_1, GH26, GH36
PUL0566 microarray, qPCR pectin Bacteroides ovatus 22205877, 29255254
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Nat Microbiol. 2018 Feb;3(2):210-219. doi: 10.1038/s41564-017-0079-1. Epub 2017 Dec 18.
2011 Dec,2018 Feb degradation 7 3 GH147, GH2, GH53
PUL0595 enzyme activity assay, qPCR, crystallization starch [Eubacterium] rectale 25388295
Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19.
2015 Jan degradation 4 1 CBM82, CBM83, GH13_41
PUL0596 enzyme activity assay, qPCR, crystallization starch [Eubacterium] rectale 25388295
Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19.
2015 Jan degradation 4 1 GH13_36
PUL0647 qPCR starch Streptococcus mutans UA159 33603728
The Route of Sucrose Utilization by Streptococcus mutans Affects Intracellular Polysaccharide Metabolism. Front Microbiol. 2021 Feb 2;12:636684. doi: 10.3389/fmicb.2021.636684. eCollection 2021.
2021 biosynthesis 5 3 CBM48, GH13_9, GT35, GT5
PUL0648 high-performance anion-exchange chromatography, substrate binding assay, thin-layer chromatography, NMR, mass spectrometry, crystallization xylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 21 CE1, CE1, CE1, CE20, CE20, CE6, GH10, GH115, GH146, GH31_4, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH43_29, GH43_29, CBM6, GH51_2, GH67, GH8, GH97
PUL0658 qPCR, growth assay beta-mannooligosaccharide Faecalibacterium prausnitzii SL3/3 34061597
Human Gut Faecalibacterium prausnitzii Deploys a Highly Efficient Conserved System To Cross-Feed on beta-Mannan-Derived Oligosaccharides. mBio. 2021 Jun 29;12(3):e0362820. doi: 10.1128/mBio.03628-20. Epub 2021 Jun 1.
2021 Jun 29 degradation 14 6 CE17, CBM35inCE17, CBM35inCE17, CE2, GH113, GH130_1, GH130_2, GH36
PUL0659 qPCR, growth assay beta-mannooligosaccharide Faecalibacterium prausnitzii SL3/3 34061597
Human Gut Faecalibacterium prausnitzii Deploys a Highly Efficient Conserved System To Cross-Feed on beta-Mannan-Derived Oligosaccharides. mBio. 2021 Jun 29;12(3):e0362820. doi: 10.1128/mBio.03628-20. Epub 2021 Jun 1.
2021 Jun 29 degradation 2 2 GH3
PUL0662 thin-layer chromatography, liquid chromatography and mass spectrometry, qPCR, clone and expression beta-mannan Phocaeicola dorei DSM 17855 34339781
BdPUL12 depolymerizes beta-mannan-like glycans into mannooligosaccharides and mannose, which serve as carbon sources for Bacteroides dorei and gut probiotics. Int J Biol Macromol. 2021 Sep 30;187:664-674. doi: 10.1016/j.ijbiomac.2021.07.172. Epub 2021 Jul 31.
2021 Sep 30 degradation 8 4 CE7, GH130_1, GH26, GH5_7
PUL0671 gene deletion mutant and growth assay, enzyme activity assay, Western Blot, qPCR cellulose Cytophaga hutchinsonii ATCC 33406 34731049
A Type IX Secretion System Substrate Involved in Crystalline Cellulose Degradation by Affecting Crucial Cellulose Binding Proteins in Cytophaga hutchinsonii. Appl Environ Microbiol. 2022 Jan 25;88(2):e0183721. doi: 10.1128/AEM.01837-21. Epub 2021 Nov 3.
2022 Jan 25 degradation 6 0 NA
PUL0673 NMR, substrate binding assay, liquid chromatography and mass spectrometry human milk oligosaccharide Bifidobacterium pseudocatenulatum DSM20438 34757822
Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10.
2022 Jan 25 degradation 8 1 GH95
PUL0712 growth assay, RNA-seq, qPCR pectic polysaccharide Bacteroides thetaiotaomicron VPI-5482 37451376
A pectic polysaccharide isolated from Achyranthes bidentata is metabolized by human gut Bacteroides spp. Int J Biol Macromol. 2023 Sep 1;248:125785. doi: 10.1016/j.ijbiomac.2023.125785. Epub 2023 Jul 13.
2023 Sep 1 degradation 10 0 NA
PUL0713 growth assay, RNA-seq, qPCR pectic polysaccharide Bacteroides thetaiotaomicron VPI-5482 37451376
A pectic polysaccharide isolated from Achyranthes bidentata is metabolized by human gut Bacteroides spp. Int J Biol Macromol. 2023 Sep 1;248:125785. doi: 10.1016/j.ijbiomac.2023.125785. Epub 2023 Jul 13.
2023 Sep 1 degradation 4 0 NA