| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0001 | RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 15 | 9 | CE17, CBM35inCE17, CE2, GH1, GH113, GH130_1, GH130_2, GH36 |
| PUL0051 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Cellulophaga lytica | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 6 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0052 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Maricaulis maris | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 10 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0053 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Stenotrophomonas maltophilia | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 8 | 2 | PL17_2, PL17, PL6 |
| PUL0054 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Alteromonas macleodii | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 9 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0055 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides sp. 1_1_30 | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 7 | 2 | PL17_2, PL17, PL6, PL6_1 |
| PUL0056 | sequence homology analysis, NMR, size-exclusion chromatography (SEC), clone and expression, recombinant protein expression, enzyme kinetic analysis | alginate | Bacteroides eggerthii | 29795267 Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1. |
2018 May 23 | degradation | 5 | 3 | CE20, PL17_2, PL17, PL6, PL6_1 |
| PUL0099 | RNA-seq, substrate binding assay, enzyme activity assay, mass spectrometry | beta-mannan | Roseburia intestinalis | 30796211 The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary beta-mannans. Nat Commun. 2019 Feb 22;10(1):905. doi: 10.1038/s41467-019-08812-y. |
2019 Feb 22 | degradation | 3 | 3 | CBM27, GH26, CBM23, GH3 |
| PUL0160 | mass spectrometry, sequence homology analysis | alpha-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 35 | 12 | AA3, CBM32, GH125, GH2, GH43_34, GH76, GH92 |
| PUL0161 | mass spectrometry, sequence homology analysis, gene deletion mutant and growth assay, microarray, qPCR | alpha-mannan | Bacteroides thetaiotaomicron | 30246424, 25567280, 18996345 Alpha- and beta-mannan utilization by marine Bacteroidetes. Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2018 Nov,2015 Jan 8,2008 Nov 13 | degradation | 26 | 6 | GH125, GH67, GH76, GH92, GH97 |
| PUL0162 | mass spectrometry, sequence homology analysis | alpha-mannan | Bacteroides thetaiotaomicron | 30246424, 18996345 Alpha- and beta-mannan utilization by marine Bacteroidetes. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2018 Nov,2008 Nov 13 | degradation | 13 | 2 | GH92, GH99 |
| PUL0163 | mass spectrometry, sequence homology analysis, microarray, qPCR | alpha-mannan | Bacteroides thetaiotaomicron | 30246424, 18996345 Alpha- and beta-mannan utilization by marine Bacteroidetes. Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. |
2018 Nov,2008 Nov 13 | degradation | 21 | 9 | GH125, GH130_3, GH38, CBM32, GH76, GH92, GT32 |
| PUL0164 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Leeuwenhoekiella sp. MAR_2009_132 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 19 | 12 | CBM8, CE2, CE20, GH130_1, GH26, GH27, GH3, GH5_2, GH5_7, GH9 |
| PUL0165 | mass spectrometry, sequence homology analysis, differential gene expression | beta-mannan | Salegentibacter sp. Hel_I_6 | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 16 | 8 | CE20, GH130_1, GH26, GH27, GH30, GH5_2, GH9 |
| PUL0167 | mass spectrometry, sequence homology analysis | beta-mannan | Bacteroides ovatus | 30246424 Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16. |
2018 Nov | degradation | 12 | 4 | GH130_1, GH26, GH36 |
| PUL0220 | mass spectrometry, target decoy database analysis | beta-glucan | Polaribacter sp. Hel1_33_49 | 25478683 Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5. |
2015 Jun | degradation | 11 | 5 | GH149, GH16_3, GH17, GH3, GH30_1 |
| PUL0224 | RT-PCR, qRT-PCR, ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 23418511, 20013800 A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2013,2010 Feb | degradation | 16 | 14 | CE1, CBM6, GH10, CBM6, GH146, CBM22, GH27, CBM6, GH2, CBM6, GH30_8, CBM6, GH43_10, CBM91, CBM6, GH43_16, CBM6, GH43_29, CBM6, GH59, CBM6, GH62, CBM6, GH62, CBM6, CE6, GH95, CBM32, CBM6 |
| PUL0234 | proteome fractionation, mass spectrometry, target decoy database analysis | beta-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 7 | 3 | GH16_3, GH3 |
| PUL0235 | proteome fractionation, mass spectrometry, target decoy database analysis | alginate | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 20 | 6 | PL17_2, PL17, PL6, PL6_1, PL7, PL7_5 |
| PUL0236 | proteome fractionation, mass spectrometry, target decoy database analysis | alpha-glucan | Gramella forsetii | 24522261 Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13. |
2014 Jul | degradation | 13 | 4 | GH13, GH13_46, GH13_7, GH65 |
| PUL0320 | liquid chromatography and mass spectrometry, mass spectrometry, target decoy database analysis | cellulose | Caldicellulosiruptor bescii | 29475869, 29588665, 21227922 Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses. The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate. Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Appl Environ Microbiol. 2018 Apr 16;84(9):e02694-17. doi: 10.1128/AEM.02694-17. Print 2018 May 1. Biotechnol Biofuels. 2018 Mar 23;11:80. doi: 10.1186/s13068-018-1076-1. eCollection 2018. Nucleic Acids Res. 2011 Apr;39(8):3240-54. doi: 10.1093/nar/gkq1281. Epub 2011 Jan 11. |
2018 May 1,2018,2011 Apr | degradation | 19 | 10 | CBM66, PL3_1, CBM66, PL9_1, GH10, CBM3, CBM3, GH48, GH5_8, CBM3, CBM3, CBM3, GH5_1, GH5_8, CBM3, CBM3, GH44, GH74, GH74, GH74, GH74, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH48, GH9, CBM3, CBM3, CBM3, GH5_8, GT39, PL11, CBM3 |
| PUL0327 | microarray, gas chromatography, mass spectrometry, gene deletion mutant and growth assay, complementation study | starch | Enterococcus faecalis | 28455338, 32680872 Enzymes Required for Maltodextrin Catabolism in Enterococcus faecalis Exhibit Novel Activities. Enterococcus faecalis Maltodextrin Gene Regulation by Combined Action of Maltose Gene Regulator MalR and Pleiotropic Regulator CcpA. Appl Environ Microbiol. 2017 Jun 16;83(13):e00038-17. doi: 10.1128/AEM.00038-17. Print 2017 Jul 1. Appl Environ Microbiol. 2020 Sep 1;86(18):e01147-20. doi: 10.1128/AEM.01147-20. Print 2020 Sep 1. |
2017 Jul 1,2020 Sep 1 | degradation | 6 | 2 | CBM34, GH13_20, GH13_31 |
| PUL0328 | microarray, gas chromatography, mass spectrometry | xylan | Gramella flava | 28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. |
2017 | degradation | 10 | 5 | GH127, GH2, GH43, GH43_26, GH5_13 |
| PUL0329 | microarray, gas chromatography, mass spectrometry | xylan | Gramella flava | 28261179 Characterization of Potential Polysaccharide Utilization Systems in the Marine Bacteroidetes Gramella Flava JLT2011 Using a Multi-Omics Approach. Front Microbiol. 2017 Feb 14;8:220. doi: 10.3389/fmicb.2017.00220. eCollection 2017. |
2017 | degradation | 25 | 9 | CE15, CE20, CE20, GH10, GH115, GH3, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH67 |
| PUL0367 | mass spectrometry | galactooligosaccharide | Bifidobacterium longum | 18539808 Differential transcriptional response of Bifidobacterium longum to human milk, formula milk, and galactooligosaccharide. Appl Environ Microbiol. 2008 Aug;74(15):4686-94. doi: 10.1128/AEM.00122-08. Epub 2008 Jun 6. |
2008 Aug | degradation | 3 | 1 | GH42 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high-performance anion-exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800, 8936327 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013. |
2010 Feb,1996 Apr | degradation | 12 | 10 | CBM4, GH9, GH48, GH5_1, GH5_17, GH8, GH9, GH9, CBM3, PL11 |
| PUL0435 | mass spectrometry, high-performance anion-exchange chromatography | beta-glucan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 13 | 4 | GH16_3, GH30_1, GH30_3, GH43_34 |
| PUL0436 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 8 | 3 | CBM32, CBM32, GH13_46, GH65 |
| PUL0437 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 1 | GH51_5 |
| PUL0438 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 7 | 4 | CE1, GH158, GH89, GT2, GH26, GH158 |
| PUL0439 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 4 | 1 | GH33 |
| PUL0440 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 5 | 2 | GH2, GH76 |
| PUL0441 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH130_3, GH43_26, CBM13 |
| PUL0442 | mass spectrometry, high-performance anion-exchange chromatography, SDS-PAGE, recombinant protein expression, enzyme activity assay, substrate binding assay | glucomannan/chitin | Chitinophaga pinensis DSM 2588 | 28069559, 37493618 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. A polysaccharide utilization locus from Chitinophaga pinensis simultaneously targets chitin and beta-glucans found in fungal cell walls. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. mSphere. 2023 Aug 24;8(4):e0024423. doi: 10.1128/msphere.00244-23. Epub 2023 Jul 26. |
2017 Mar 6,2023 Aug 24 | degradation | 9 | 3 | GH16_3, CBM6, GH18, CBM6, GH18, GH18, CBM5 |
| PUL0443 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 9 | 3 | CBM6, CBM6, GH16_3, CBM32, GH16_3, CBM92 |
| PUL0444 | mass spectrometry, high-performance anion-exchange chromatography | glucomannan | Chitinophaga pinensis | 28069559 Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6. |
2017 Mar 6 | degradation | 6 | 2 | GH19_2, GH31_4 |
| PUL0459 | RNA-seq, analysis of reaction products, enzyme activity assay, thin-layer chromatography, liquid chromatography, mass spectrometry | agarose | Colwellia echini A3 | 31915221, 33811026 A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19. Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26. |
2020 Jan 8,2021 May 26 | degradation | 45 | 9 | GH117, GH117, GH2, GH29, GH50, GH86, GH96 |
| PUL0582 | NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay | human milk oligosaccharide | Lactococcus lactis | 22660716 A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1. |
2012 Aug | degradation | 5 | 2 | GH1, GH170 |
| PUL0648 | high-performance anion-exchange chromatography, substrate binding assay, thin-layer chromatography, NMR, mass spectrometry, crystallization | xylan | Dysgonomonas mossii DSM 22836 | 33667545 A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2. |
2021 Jan-Jun | degradation | 37 | 21 | CE1, CE1, CE1, CE20, CE20, CE6, GH10, GH115, GH146, GH31_4, GH43_1, GH43_10, CBM91, GH43_12, CBM91, GH43_29, GH43_29, CBM6, GH51_2, GH67, GH8, GH97 |
| PUL0650 | enzyme activity assay, high-performance anion-exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay | arabinogalactan | Bifidobacterium longum JCM 7052 | 33674431 Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27. |
2021 Apr 27 | degradation | 7 | 2 | GH36, GH39, CBM35 |
| PUL0651 | enzyme activity assay, NMR | agarose | Gilvimarinus chinensis DSM 19667 | 33691998 Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19. |
2021 Aug 1 | degradation | 63 | 15 | CBM6, CBM6, CBM6, GH86, GH86, CE1, GH117, GH127, GH16_16, CBM13, GH16_16, CBM6, CBM6, GH16_3, GH167, GH2, GH50, GH86 |
| PUL0657 | recombinant protein expression, NMR | levoglucosan | Bacillus smithii S-2701M | 33208778 Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction. Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8. |
2020 Nov 18 | degradation | 5 | 2 | GH109, GH179 |
| PUL0673 | NMR, substrate binding assay, liquid chromatography and mass spectrometry | human milk oligosaccharide | Bifidobacterium pseudocatenulatum DSM20438 | 34757822 Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10. |
2022 Jan 25 | degradation | 8 | 1 | GH95 |
| PUL0674 | microarray, enzyme activity assay, high-performance anion-exchange chromatography, mass spectrometry, RNA-seq, affinity gel electrophoresis, carbohydrate binding assay, microscale thermophoresis | beta-glucan | Bacteroides ovatus ATCC 8483 | 34817219, 29020628, 22205877, 32801182, 31062073 Mapping Molecular Recognition of beta1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus. Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Microbiol Spectr. 2021 Dec 22;9(3):e0182621. doi: 10.1128/Spectrum.01826-21. Epub 2021 Nov 24. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20):e01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. |
2021 Dec 22,2017 Oct 10,2011 Dec,2020 Oct 1,2019 Nov | degradation | 8 | 3 | GH16_3, GH3 |
| PUL0681 | enzyme activity assay, NMR | pectic polysaccharide | Bacteroidaceae bacterium | 35110564 Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides. Nat Commun. 2022 Feb 2;13(1):629. doi: 10.1038/s41467-022-28310-y. |
2022 Feb 2 | degradation | 10 | 5 | GH173, GH2, GH5_57, GH78 |
| PUL0722 | RNA-seq, mass spectrometry, SDS-PAGE, isothermal titration calorimetry (ITC), high-performance anion-exchange chromatography, enzyme kinetic analysis, thin-layer chromatography | xylan | Polaribacter sp. Q13 | 38169280 The catabolic specialization of the marine bacterium Polaribacter sp. Q13 to red algal beta1,3/1,4-mixed-linkage xylan. Appl Environ Microbiol. 2024 Jan 24;90(1):e0170423. doi: 10.1128/aem.01704-23. Epub 2024 Jan 3. |
2024 Jan 24 | degradation | 30 | 9 | CBM4, CBM4, GH10, GH26, GH3, GH43_1, GH43_12, CBM91 |
| PUL0725 | NMR, clone and expression, enzyme kinetic analysis | alpha-glucan | Marinovum sp. | 38180643 A Broad-Spectrum alpha-Glucosidase of Glycoside Hydrolase Family 13 from Marinovum sp., a Member of the Roseobacter Clade. Appl Biochem Biotechnol. 2024 Sep;196(9):6059-6071. doi: 10.1007/s12010-023-04820-3. Epub 2024 Jan 5. |
2024 Sep | degradation | 9 | 1 | GH13_23 |
| PUL0726 | reducing-sugar assay, NMR, clone and expression, liquid chromatography and mass spectrometry, mass spectrometry | sulfosugar | Agrobacterium tumefaciens str. C58 | 35074914 Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria. Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2116022119. doi: 10.1073/pnas.2116022119. |
2022 Jan 25 | degradation | 9 | 1 | GH31_13 |
| PUL0727 | SDS-PAGE, NMR, enzyme activity assay, size-exclusion chromatography (SEC) | fucoidan | Wenyingzhuangia fucanilytica strain CZ1127 | 38203394 The Discovery of the Fucoidan-Active Endo-1-->4-alpha-L-Fucanase of the GH168 Family, Which Produces Fucoidan Derivatives with Regular Sulfation and Anticoagulant Activity. Int J Mol Sci. 2023 Dec 22;25(1):218. doi: 10.3390/ijms25010218. |
2023 Dec 22 | degradation | 30 | 16 | GH107, GH117, GH141, GH168, GH29, GH43_2, GH95 |
| PUL0730 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga algicola DSM 14237 | 38442258 Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 17 | 3 | GH127, GH16_13 |
| PUL0731 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Saccharicrinis fermentans DSM 9555 | 38442258 Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 20 | 5 | GH110, GH127, GH167, GH2 |
| PUL0732 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga baltica 18 | 38442258 Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 19 | 3 | GH127, GH16_13 |
| PUL0733 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Echinicola pacifica DSM 19836 | 38442258 Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 16 | 2 | GH127, GH129 |
| PUL0734 | enzyme activity assay, recombinant protein expression, NMR, HPLC, clone and expression | carrageenan | Cellulophaga lytica DSM 7489 | 38442258 Biocatalytic Conversion of Carrageenans for the Production of 3,6-Anhydro-D-galactose. J Agric Food Chem. 2024 Mar 20;72(11):5816-5827. doi: 10.1021/acs.jafc.3c08613. Epub 2024 Mar 5. |
2024 Mar 20 | degradation | 20 | 3 | GH127, GH129, GH2 |
| PUL0737 | mass spectrometry, SDS-PAGE, growth assay | arabinogalactan | Maribacter sp. MAR_2009_72 | 38569650 Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72. FEMS Microbiol Ecol. 2024 Apr 10;100(5):fiae045. doi: 10.1093/femsec/fiae045. |
2024 Apr 10 | degradation | 16 | 4 | CE20, CE20, GH10, GH43_1, GH67 |
| PUL0738 | mass spectrometry, SDS-PAGE, growth assay | arabinogalactan | Maribacter sp. MAR_2009_72 | 38569650 Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72. FEMS Microbiol Ecol. 2024 Apr 10;100(5):fiae045. doi: 10.1093/femsec/fiae045. |
2024 Apr 10 | degradation | 50 | 14 | CBM67, GH78, CE12, CE12, CE12, GH105, GH106, GH115, GH179, GH2, GH28, GH29, GH31_9, GH33, GH43_18 |
| PUL0739 | mass spectrometry, SDS-PAGE, growth assay | arabinogalactan | Maribacter sp. MAR_2009_72 | 38569650 Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72. FEMS Microbiol Ecol. 2024 Apr 10;100(5):fiae045. doi: 10.1093/femsec/fiae045. |
2024 Apr 10 | degradation | 56 | 12 | CE12, GH105, GH140, GH177, GH179, GH28, GH43_10, CBM91, GH43_19, GH43_34, GH51_1, PL10_1, CE8, PL1_2 |
| PUL0750 | RNA-seq, BCA assay, pNP glycoside assay, HPAEC-PAD, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | beta-glucan | Segatella copri DSM 18205 | 39122003, 39636128 The molecular basis of cereal mixed-linkage beta-glucan utilization by the human gut bacterium Segatella copri. Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. J Biol Chem. 2024 Sep;300(9):107625. doi: 10.1016/j.jbc.2024.107625. Epub 2024 Aug 8. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2024 Sep,2025 Jan 31 | degradation | 11 | 3 | GH3, GH5_4, GH94 |
| PUL0759 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 3 | 2 | GH2, GH31_4 |
| PUL0760 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 7 | 3 | GH5_4, GH5_7 |
| PUL0761 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 10 | 4 | GH10, GH35, GH43_1, GH67 |
| PUL0762 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 9 | 3 | GH10, GH43_12, CBM91, GH5_21 |
| PUL0763 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 5 | 3 | CE6, CE1, GH31_4, GH43_2, CBM6, GH8 |
| PUL0764 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | Hemicellulose | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 4 | 4 | GH43_10, CBM91, GH43_29, CBM6, GH43_29, CBM6, GH43_10, CBM91, GH95 |
| PUL0765 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | inulin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 10 | 1 | GH32 |
| PUL0766 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | starch | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 5 | 2 | GH13_44, GH97 |
| PUL0767 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 15 | 6 | CE8, GH28, GH28, GH105, GH43_10, CBM91, GH95, PL1_2 |
| PUL0768 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 7 | 3 | GH2, GH53, PL1, CBM77 |
| PUL0769 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 9 | 3 | GH43_4, GH43_5, GH51_2 |
| PUL0770 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | pectin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 8 | 1 | GH51_1 |
| PUL0793 | enzyme activity assay, quantification of reaction product reducing ends, RNA-seq, differential gene expression, NMR, MALDI-TOF/MS, gas chromatography, mass spectrometry, bicinchoninic acid (BCA) assay, recombinant protein expression | arabinan | Bacteroides intestinalis DSM 17393 | 39443715 In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides. Nat Chem Biol. 2025 Apr;21(4):544-554. doi: 10.1038/s41589-024-01763-6. Epub 2024 Oct 23. |
2025 Apr | degradation | 14 | 6 | CE1, GH127, GH146, GH43_34, CBM32, GH97 |
| PUL0794 | enzyme activity assay, quantification of reaction product reducing ends, RNA-seq, differential gene expression, NMR, MALDI-TOF/MS, gas chromatography, mass spectrometry, bicinchoninic acid (BCA) assay, recombinant protein expression | arabinan | Bacteroides intestinalis DSM 17393 | 39443715 In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides. Nat Chem Biol. 2025 Apr;21(4):544-554. doi: 10.1038/s41589-024-01763-6. Epub 2024 Oct 23. |
2025 Apr | degradation | 23 | 8 | GH146, GH28, GH43_29, GH43_4, GH51_1, GH51_2, GH97 |
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