Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry (ITC) beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2):e00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 GH158, GH16_3, GH3
PUL0335 fosmid library screen, enzyme activity assay, thin-layer chromatography xylan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 31 5 GH13_46, GH158, GH16_3, GH3, GH97
PUL0336 fosmid library screen, enzyme activity assay, thin-layer chromatography xylan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 25 4 GH158, GH16_3, GH3, GT2
PUL0337 fosmid library screen, enzyme activity assay, thin-layer chromatography xylan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 29 4 GH158, GH16_3, GH3, GT2
PUL0338 fosmid library screen, enzyme activity assay, thin-layer chromatography xylan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 34 5 GH158, GH16_3, GH3, GH97, GT2
PUL0438 mass spectrometry, high-performance anion-exchange chromatography glucomannan Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 7 4 CE1, GH158, GH89, GT2, GH26, GH158
PUL0646 recombinant protein expression, crystallization, affinity gel electrophoresis, isothermal titration calorimetry (ITC) beta-glucan Bacteroides fluxus YIT 12057 33587952
Distinct protein architectures mediate species-specific beta-glucan binding and metabolism in the human gut microbiota. J Biol Chem. 2021 Jan-Jun;296:100415. doi: 10.1016/j.jbc.2021.100415. Epub 2021 Feb 13.
2021 Jan-Jun degradation 6 2 GH158, GH3