| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0008 | enzyme activity assay, qPCR, thin-layer chromatography, substrate binding assay | fructan | Bacteroides thetaiotaomicron | 28103254, 25841008 A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. Differential Metabolism of Exopolysaccharides from Probiotic Lactobacilli by the Human Gut Symbiont Bacteroides thetaiotaomicron. PLoS One. 2017 Jan 19;12(1):e0169989. doi: 10.1371/journal.pone.0169989. eCollection 2017. Appl Environ Microbiol. 2015 Jun 15;81(12):3973-83. doi: 10.1128/AEM.00149-15. Epub 2015 Apr 3. |
2017,2015 Jun 15 | degradation | 12 | 3 | GH32 |
| PUL0024 | enzyme activity assay, qPCR, carbohydrate binding assay | fructan | uncultured bacterium | 31915220 Harvesting of Prebiotic Fructooligosaccharides by Nonbeneficial Human Gut Bacteria. mSphere. 2020 Jan 8;5(1):e00771-19. doi: 10.1128/mSphere.00771-19. |
2020 Jan 8 | degradation | 12 | 1 | GH32 |
| PUL0219 | sugar utilization assay, enzyme activity assay | fructan | Lactobacillus paracasei | 17644636 Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195. Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20. |
2007 Sep | degradation | 7 | 1 | GH32 |
| PUL0277 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 5 | 1 | GH32 |
| PUL0278 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0279 | gene deletion mutant and growth assay, qRT-PCR | fructan | Streptococcus pneumoniae | 23264576 The ABC transporter encoded at the pneumococcal fructooligosaccharide utilization locus determines the ability to utilize long- and short-chain fructooligosaccharides. J Bacteriol. 2013 Mar;195(5):1031-41. doi: 10.1128/JB.01560-12. Epub 2012 Dec 21. |
2013 Mar | degradation | 6 | 1 | GH32 |
| PUL0394 | microarray | fructan | Roseburia inulinivorans | 20679207 Substrate-driven gene expression in Roseburia inulinivorans: importance of inducible enzymes in the utilization of inulin and starch. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4672-9. doi: 10.1073/pnas.1000091107. Epub 2010 Aug 2. |
2011 Mar 15 | degradation | 6 | 1 | GH32 |
| PUL0425 | microarray | fructan | Bacteroides ovatus | 22205877 Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. |
2011 Dec | degradation | 11 | 4 | GH32, GH91 |
| PUL0569 | clone and expression, enzyme activity assay, Northern Blot | fructan | Bacillus subtilis | 11739774 yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis. Microbiology (Reading). 2001 Dec;147(Pt 12):3413-9. doi: 10.1099/00221287-147-12-3413. |
2001 Dec | degradation | 4 | 2 | GH32, GH68_1 |
| PUL0581 | SDS-PAGE, enzyme activity assay | fructan | Microbulbifer sp. JAM-3301 | 22286980 Cloning and sequencing of inulinase and beta-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes. Appl Environ Microbiol. 2012 Apr;78(7):2493-5. doi: 10.1128/AEM.07442-11. Epub 2012 Jan 27. |
2012 Apr | degradation | 3 | 2 | GH32 |
| PUL0587 | RT-PCR, enzyme activity assay | fructan | Prevotella intermedia | 23266804 Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia. Gene. 2013 Feb 25;515(2):291-7. doi: 10.1016/j.gene.2012.12.023. Epub 2012 Dec 22. |
2013 Feb 25 | degradation | 3 | 1 | GH32 |
| PUL0632 | recombinant protein expression | fructan | Roseburia inulinivorans DSM 16841 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0633 | recombinant protein expression | fructan | Roseburia faecis M72 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0634 | recombinant protein expression | fructan | Eubacterium rectale ATCC 33656 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 6 | 1 | GH32 |
| PUL0635 | recombinant protein expression | fructan | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 7 | 1 | GH32 |
| PUL0636 | recombinant protein expression | fructan | Coprococcus eutactus JCM 31265 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 9 | 1 | GH32 |
| PUL0637 | recombinant protein expression | fructan | Faecalibacterium prausnitzii A2165 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0638 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 5 | 1 | GH32 |
| PUL0639 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 4 | 2 | GH32 |
| PUL0640 | recombinant protein expression | fructan | Anaerostipes hadrus DSM 3319 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | CBM66, GH32, CBM66 |
| PUL0641 | recombinant protein expression | fructan | Anaerostipes caccae L1-92 DSM 14662 | 33439065 Characterization of fructooligosaccharide metabolism and fructooligosaccharide-degrading enzymes in human commensal butyrate producers. Gut Microbes. 2021 Jan-Dec;13(1):1-20. doi: 10.1080/19490976.2020.1869503. |
2021 Jan-Dec | degradation | 10 | 1 | GH32 |
| PUL0678 | RNA-seq, thin-layer chromatography, growth assay | inulin | Lactiplantibacillus plantarum QS7T | 34980384 Global genome and comparative transcriptomic analysis reveal the inulin consumption strategy of Lactiplantibacillus plantarum QS7T. Food Res Int. 2022 Jan;151:110846. doi: 10.1016/j.foodres.2021.110846. Epub 2021 Dec 2. |
2022 Jan | degradation | 5 | 2 | GH32, GH36 |
| PUL0679 | RNA-seq, thin-layer chromatography, growth assay | inulin | Lactiplantibacillus plantarum QS7T | 34980384 Global genome and comparative transcriptomic analysis reveal the inulin consumption strategy of Lactiplantibacillus plantarum QS7T. Food Res Int. 2022 Jan;151:110846. doi: 10.1016/j.foodres.2021.110846. Epub 2021 Dec 2. |
2022 Jan | degradation | 7 | 1 | GH32 |
| PUL0765 | RNA-seq, qRT-PCR, gas chromatography, mass spectrometry, thin-layer chromatography | inulin | Segatella copri DSM 18205 | 39636128 Transcriptional delineation of polysaccharide utilization loci in the human gut commensal Segatella copri DSM18205 and co-culture with exemplar Bacteroides species on dietary plant glycans. Appl Environ Microbiol. 2025 Jan 31;91(1):e0175924. doi: 10.1128/aem.01759-24. Epub 2024 Dec 5. |
2025 Jan 31 | degradation | 10 | 1 | GH32 |
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