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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0160

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis

Genomic accession number

NZ_JQNQ01000001.1

Nucelotide position range

1815119-1861584

Substrate

alpha-mannan

Loci

FG27_RS08030-FG27_RS08170

Species

Salegentibacter sp. Hel_I_6/1250278

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- FG27_RS08030 WP_037317819.1 0 - 927 (-) NZ_JQNQ01000001.1:1815119-1816046 5.3.1.-
- FG27_RS08035 WP_037317821.1 1109 - 2423 (-) NZ_JQNQ01000001.1:1816228-1817542 -
- FG27_RS08040 WP_081912606.1 2426 - 4796 (-) NZ_JQNQ01000001.1:1817545-1819915 3.2.1.-
- FG27_RS08045 WP_037317824.1 4837 - 7132 (-) NZ_JQNQ01000001.1:1819956-1822251 3.2.1.-
- FG27_RS08050 WP_037317827.1 7143 - 8169 (-) NZ_JQNQ01000001.1:1822262-1823288 -
- FG27_RS08055 WP_037317830.1 8184 - 8883 (-) NZ_JQNQ01000001.1:1823303-1824002 1.-.-.-
- FG27_RS08060 WP_037317834.1 8894 - 10622 (-) NZ_JQNQ01000001.1:1824013-1825741 1.-.-.-
- FG27_RS08065 WP_037317837.1 10639 - 11764 (-) NZ_JQNQ01000001.1:1825758-1826883 -
- FG27_RS08070 WP_037317840.1 11805 - 13239 (-) NZ_JQNQ01000001.1:1826924-1828358 3.2.1.163
- FG27_RS08075 WP_037317843.1 13309 - 13984 (-) NZ_JQNQ01000001.1:1828428-1829103 -
- FG27_RS08080 WP_037317846.1 14317 - 15118 (-) NZ_JQNQ01000001.1:1829436-1830237 -
- FG27_RS08085 WP_037317849.1 15132 - 16749 (-) NZ_JQNQ01000001.1:1830251-1831868 -
- FG27_RS08090 WP_081912607.1 16761 - 19824 (-) NZ_JQNQ01000001.1:1831880-1834943 -
- FG27_RS08095 WP_037317855.1 19943 - 22241 (-) NZ_JQNQ01000001.1:1835062-1837360 3.2.1.-
- FG27_RS08100 WP_037317858.1 22167 - 23112 (-) NZ_JQNQ01000001.1:1837286-1838231 -
- FG27_RS08105 WP_081912656.1 23291 - 25379 (+) NZ_JQNQ01000001.1:1838410-1840498 -
- FG27_RS08110 WP_051935788.1 25375 - 26605 (+) NZ_JQNQ01000001.1:1840494-1841724 -
- FG27_RS08115 WP_037317860.1 26949 - 28428 (+) NZ_JQNQ01000001.1:1842068-1843547 -
- FG27_RS08120 WP_037317862.1 28474 - 29302 (+) NZ_JQNQ01000001.1:1843593-1844421 -
- FG27_RS19385 WP_231563298.1 29402 - 29633 (+) NZ_JQNQ01000001.1:1844521-1844752 -
- FG27_RS19515 WP_255351525.1 29568 - 29949 (+) NZ_JQNQ01000001.1:1844687-1845068 -
- FG27_RS19520 WP_255351526.1 29887 - 30256 (+) NZ_JQNQ01000001.1:1845006-1845375 -
- FG27_RS19525 WP_255351556.1 30216 - 30546 (+) NZ_JQNQ01000001.1:1845335-1845665 -
- FG27_RS08130 WP_037317865.1 30612 - 31431 (-) NZ_JQNQ01000001.1:1845731-1846550 3.-.-.-
- FG27_RS08135 WP_051935789.1 31563 - 33768 (-) NZ_JQNQ01000001.1:1846682-1848887 3.2.1.-
- FG27_RS08140 WP_037317868.1 33788 - 34955 (-) NZ_JQNQ01000001.1:1848907-1850074 -
- FG27_RS08145 WP_037317871.1 35080 - 36112 (-) NZ_JQNQ01000001.1:1850199-1851231 -
- FG27_RS19130 WP_156101216.1 36227 - 36389 (-) NZ_JQNQ01000001.1:1851346-1851508 -
- FG27_RS19240 WP_197051675.1 36609 - 36861 (-) NZ_JQNQ01000001.1:1851728-1851980 -
- FG27_RS19245 WP_197051676.1 36863 - 37709 (-) NZ_JQNQ01000001.1:1851982-1852828 -
- FG27_RS19250 WP_197051677.1 37745 - 38696 (-) NZ_JQNQ01000001.1:1852864-1853815 -
- FG27_RS08155 WP_051935790.1 39106 - 40924 (-) NZ_JQNQ01000001.1:1854225-1856043 3.2.1.-
- FG27_RS08160 WP_037317875.1 41091 - 42870 (-) NZ_JQNQ01000001.1:1856210-1857989 5.3.1.8
- FG27_RS08165 WP_037317878.1 42952 - 45313 (-) NZ_JQNQ01000001.1:1858071-1860432 3.2.1.-
- FG27_RS08170 WP_037317881.1 45323 - 46466 (-) NZ_JQNQ01000001.1:1860442-1861585 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 927 (-) CDS No
- 1110 - 2423 (-) TC: gnl|TC-DB|A1S5F4|2.A.1.7.10 Yes
- 2427 - 4796 (-) CAZyme: GH92 Yes
- 4838 - 7132 (-) CAZyme: GH92 Yes
- 7144 - 8169 (-) CAZyme: GH43_34 Yes
- 8185 - 8883 (-) other Yes
- 8895 - 10622 (-) CAZyme: AA3 Yes
- 10640 - 11764 (-) other Yes
- 11806 - 13239 (-) CAZyme: GH125 Yes
- 13310 - 13984 (-) other Yes
- 14318 - 15118 (-) CAZyme: CBM32 Yes
- 15133 - 16749 (-) TC: gnl|TC-DB|Q8A1G2|8.A.46.1.1 Yes
- 16762 - 19824 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 19944 - 22241 (-) CAZyme: GH92 Yes
- 22168 - 23112 (-) other Yes
- 23292 - 25379 (+) CAZyme: GH92 Yes
- 25376 - 26605 (+) other Yes
- 26950 - 28428 (+) other Yes
- 28475 - 29302 (+) other Yes
- 29403 - 29633 (+) other Yes
- 29569 - 29949 (+) other Yes
- 29888 - 30256 (+) other Yes
- 30217 - 30546 (+) other Yes
- 30613 - 31431 (-) other Yes
- 31564 - 33768 (-) CAZyme: GH92 Yes
- 33789 - 34955 (-) CAZyme: GH76 Yes
- 35081 - 36112 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0044558 Yes
- 36228 - 36389 (-) other Yes
- 36610 - 36861 (-) other Yes
- 36864 - 37709 (-) other Yes
- 37746 - 38696 (-) other Yes
- 39107 - 40924 (-) CAZyme: GH2 Yes
- 41092 - 42870 (-) other Yes
- 42953 - 45313 (-) CAZyme: GH92 Yes
- 45324 - 46466 (-) TC: gnl|TC-DB|Q8A5Y0|2.A.1.7.16 Yes

PUL ID

PUL0160

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.